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Genomics, Transcriptomics and Proteomics

Semester 3

Genome organization of prokaryotes and eukaryotes. Mechanisms of genome stability, alteration and evolution. Genomic recombination. Techniques of nucleic acids analysis. DNA markers technologies. Identification of genes only recognizable by their impact in the phenotype: a) Map based cloning; b) Genome wide association studies (GWAS); c) Identification of regions of homozygosity (ROHs). Genomic and expression libraries. Genomic databases. Next generation sequencing techniques. Quantitative analysis of gene expression. From microarrays to RNAseq. Real time PCR. Epigenetic modulation. One genome multiple proteomes. Resolution of complex protein mixes through chromatographic techniques and 2D gel electrophoresis. Digestion techniques of proteins in peptides. Quantitative proteomics: Isotope (ICT) and Fluorescence (DIGE) labelling. Analysis of proteins by mass spectrometry. Identification of post-translational modifications. Protein arrays.